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Index > Protein center > Slc16a1(Gene name) > Mouse
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  • Slc16a1 (Gene name),
  • Monocarboxylate transporter 1 (Protein name ),  MOT1_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    Slc16a1(Mct1);
    Protein name:
    Monocarboxylate transporter 1(MCT 1);
    Alternative:
    Solute carrier family 16 member 1;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
    Subcellular Location:
    Cell membrane Multi-pass membrane protein
    Protein Attributes:
    Sequence length:
    493
    Sequence:
    50:
    MPPAIGGPVG | YTPPDGGWGW | AVLVGAFISI | GFSYAFPKSI | TVFFKEIEVI | 
    100:
    FSATTSEVSW | ISSIMLAVMY | AGGPISSILV | NKYGSRPVMI | AGGCLSGCGL | 
    150:
    IAASFCNTVQ | ELYLCIGVIG | GLGLAFNLNP | ALTMIGKYFY | KKRPLANGLA | 
    200:
    MAGSPVFLST | LAPLNQAFFD | IFDWRGSFLI | LGGLLLNCCV | AGSLMRPIGP | 
    250:
    EQVKLEKLKS | KESLQEAGKS | DANTDLIGGS | PKGEKLSVFQ | TINKFLDLSL | 
    300:
    FTHRGFLLYL | SGNVVMFFGL | FTPLVFLSSY | GKSKDFSSEK | SAFLLSILAF | 
    350:
    VDMVARPSMG | LAANTKWIRP | RIQYFFAASV | VANGVCHLLA | PLSTTYVGFC | 
    400:
    VYAGVFGFAF | GWLSSVLFET | LMDLIGPQRF | SSAVGLVTIV | ECCPVLLGPP | 
    450:
    LLGRLNDMYG | DYKYTYWACG | VILIIAGIYL | FIGMGINYRL | LAKEQKAEEK | 
    493:
    QKREGKEDEA | STDVDEKPKE | TMKAAQSPQQ | HSSGDPTEEE | SPV
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    KEGG:
    Pfam:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse MCT 1
    Cat.:
    E7726m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse MCT 1
    Cat.:
    E7726r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Mouse MCT 1
    Cat.:
    E7726h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Mouse MCT 1
    Cat.:
    E7726b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse MCT 1
    Cat.:
    U7726r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse MCT 1
    Cat.:
    U7726b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse MCT 1
    Cat.:
    U7726h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse MCT 1
    Cat.:
    U7726m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    Polyclonal Antibody for Mouse MCT 1
    Polyclonal Antibody for Mouse MCT 1
    Cat.:
    P7726Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse MCT 1
    Cat.:
    P7726Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse MCT 1
    Cat.:
    P7726Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Mouse MCT 1
    Monoclonal Antibody for Mouse MCT 1
    Monoclonal Antibody for Mouse MCT 1
    Monoclonal Antibody for Mouse MCT 1
    Protein for Mouse MCT 1
    Protein for Mouse MCT 1
    Protein for Mouse MCT 1
    Protein for Mouse MCT 1

    R&D Technical Data

    s

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    s
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤6.5%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of MOT1 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    102

    95-108

    Plasma

    100

    93-105

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MOT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    97-105%

    95-109%

    93-110%

    96-107%

    EDTA plasma(n=5)

    91-110%

    93-108%

    92-109%

    90-109%

    heparin plasma(n=5)

    93-106%

     

    94-106%

    95-103%

    96-104%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Cloning and sequencing of the monocarboxylate transporter from mouse Ehrlich Lettre tumour cell confirms its identity as MCT1 and demonstrates that glycosylation is not required for MCT1 function."
      Carpenter L. , Poole R.C. , Halestrap A.P.
      Biochim. Biophys. Acta1279:157-163(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];SUBCELLULAR LOCATION;TISSUE SPECIFICITY
    2. 2.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];TISSUE SPECIFICITY
      strain: 129.
      tissue: Kidney.
    3. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: FVB/N.
      tissue: Mammary gland.
    4. 4.
      "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P. , Tweedie-Cullen R. , Livingstone-Zatchej M. , Weinandy F. , Waidelich M. , Longo D. , Gehrig P. , Potthast F. , Rutishauser D. , Gerrits B. , Panse C. , Schlapbach R. , Mansuy I.M.
      Mol. Cell. Proteomics6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Brain cortex.
    5. 5.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-210; SER-213; THR-224 AND SER-491;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Liver.
    6. 6.
      "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H. , Ye M. , Dong J. , Han G. , Jiang X. , Wu R. , Zou H.
      J. Proteome Res.7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Liver.
    7. 7.
      "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M. , English L. , Lemieux S. , Courcelles M. , Desjardins M. , Thibault P.
      Immunity30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213; SER-230; SER-461; THR-462; SER-477 AND SER-491;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    8. 8.
      "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M. , Bailey C.M. , Cunningham D.L. , Heath J.K. , Cooper H.J.
      Mol. Cell. Proteomics8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Embryonic fibroblast.
    9. 9.
      "Overexpression of monocarboxylate transporter-1 (SLC16A1) in mouse pancreatic beta-cells leads to relative hyperinsulinism during exercise."
      Pullen T.J. , Sylow L. , Sun G. , Halestrap A.P. , Richter E.A. , Rutter G.A.
      Diabetes61:1719-1725(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;TISSUE SPECIFICITY
    10. 10.
      "The monocarboxylate transporter family--role and regulation."
      Halestrap A.P. , Wilson M.C.
      IUBMB Life64:109-119(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
    11. 11.
      "Basigin interacts with both MCT1 and MCT2 in murine spermatozoa."
      Mannowetz N. , Wandernoth P. , Wennemuth G.
      J. Cell. Physiol.227:2154-2162(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH BSG;TISSUE SPECIFICITY;SUBCELLULAR LOCATION
    12. 12.
      "Resistance to diet-induced obesity and associated metabolic perturbations in haploinsufficient monocarboxylate transporter 1 mice."
      Lengacher S. , Nehiri-Sitayeb T. , Steiner N. , Carneiro L. , Favrod C. , Preitner F. , Thorens B. , Stehle J.C. , Dix L. , Pralong F. , Magistretti P.J. , Pellerin L.
      PLoS ONE8:E82505-E82505(2013) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE;FUNCTION;TISSUE SPECIFICITY
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